Composite

Part:BBa_K2911003:Design

Designed by: Ryan Lambert   Group: iGEM19_USAFA   (2019-10-08)


Hybrid Vector for E. Coi and rhodococcus with prmA Promoter Sequence and mRFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal SpeI site found at 3107
    Illegal PstI site found at 3125
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 3107
    Illegal PstI site found at 3125
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal SpeI site found at 3107
    Illegal PstI site found at 3125
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 3107
    Illegal PstI site found at 3125
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 1031
    Illegal NgoMIV site found at 2207
    Illegal NgoMIV site found at 2218
    Illegal NgoMIV site found at 4960
    Illegal AgeI site found at 2817
    Illegal AgeI site found at 2929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6120
    Illegal SapI.rc site found at 597
    Illegal SapI.rc site found at 807
    Illegal SapI.rc site found at 1964


Design Notes

Hybrid Vector for both E. Coli and Rhodoccocus


Source

Plasmid derived from pSRK21. Bought from Addgene.

References